Bafuputsi ba sebelisitse phala e ncha e tsitsitseng ea isotope (HT-SIP) le metagenomics ho fumana pono ea pele ea microbiome e sebetsang e potolohileng semela se molemo sa symbiont, arbuscular mycorrhizal fungus (AMF). Mokitlane: Lawrence Livermore National Laboratory
Ho hokahanya boitsebahatso ba likokoana-hloko tse hlaha le litšobotsi tsa tsona tsa 'mele le mesebetsi ea tikoloho ke sepheo sa mantlha sa litsebi tsa tikoloho. Har'a mekhoa e loanelang sepheo sena, Stable Isotope Probing-SIP-e nkoa e le eona e sebetsang ka ho fetisisa bakeng sa ho ithuta likokoana-hloko tse sebetsang libakeng tsa tlhaho.
Bo-rasaense ba Lawrence Livermore National Laboratory (LLNL) ba thehile mokhoa o mocha oa SIP-high-throughput SIP-o iketsetsang mehato e mengata ea ho hlahloba isotope e tsitsitseng, e lumellang lipatlisiso tsa ts'ebetso ea likokoana-hloko tsa likokoana-hloko tlas'a maemo a sebele, ntle le tlhokahalo ea lab culturing.
Ho SIP, likokoana-hloko tse sebetsang li khetholloa ka ho kenngoa ha li-isotopi tse tsitsitseng ka har'a biomass ea tsona. Ke e 'ngoe ea mekhoa e matla ka ho fetisisa ho ecology ea likokoana-hloko kaha e khona ho khetholla likokoana-hloko tse sebetsang le litšobotsi tsa tsona tsa bophelo (tšebeliso ea substrate, cellular biochemistry, metabolism, kholo, lefu) metseng e rarahaneng tlas'a maemo a tlhaho.
Ka tloaelo, mokhoa oa SIP o hloka mosebetsi o boima oa matsoho 'me o lumella palo e fokolang ea lisampole. Empa mokhoa o mocha oa LLNL o hloka karolo ea botšelela ea palo ea mosebetsi oa matsoho ha o bapisoa le buka ea SIP mme o lumella lisampole tse 16 hore li sebetsanoe ka nako e le 'ngoe.
"Mokhoa oa rona oa boits'oaro o fokotsa nako ea opereishene mme o ntlafatsa ho ikatisa ka ho shebana le mehato e matla ka ho fetesisa ea SIP," ho boletse rasaense oa LLNL Erin Nuccio, le sengoli se etelletseng pele sa pampiri e hlahang koranteng ea Microbiome. "Joale re sebelisitse mokhoa ona ho sebetsana le lisampole tse fetang sekete, ho kenyeletsoa tse ling tse tsoang libakeng tse sa rutehang haholo tsa mobu."
E 'ngoe ea li-microhabitat tse joalo ke mobu o pota-potileng lisele tsa mycorrhizae-mofuta oa li-fungus tse etsang likamano tsa symbiotic le 72% ea limela tsohle tsa mobu. E le phapanyetsano bakeng sa khabone ea semela, fungus (arbuscular mycorrhizal fungus) e fa mabotho a eona lisebelisoa tsa bohlokoa tse kang nitrogen, phosphorus le metsi.
Thutong ena ea bopaki ba khopolo, bangoli ba bontšitse "tepo ea lijo" ea litšebelisano tse hlohlellelitsoeng ke li-fungus tsa mycorrhizal mobung.
"Re nahana hore ena ke tsela e kholo ea hore na k'habone ea semela e ajoa joang mobung. Mobu o na le letamo le leholo ka ho fetisisa la ho palama libaesekele lefatšeng ka bophara," ho boletse sengoli se tsamaisanang le eena Jennifer Pett-Ridge, eo e leng moetapele oa morero oa LLNL le hlooho ea Ofisi ea Saense ea Lefapha la Matla "Microbes Persist" Soil Microbiome Scientific Focus Area. . "Re ile ra tatellana le palo e nyane ea DNA, ra tseba hore na ke lintho life tse phelang, 'me ra etsa liphatsa tsa lefutso le litšebelisano tse ka bang teng."
Bangoli ba bang ba LLNL ba kenyelletsa Steven Blazewicz, Marissa Lafler, Ashley Campbell, Jeffrey Kimbrel, Jessica Wollard, Rachel Hestrin hammoho le bafuputsi ba Lawrence Berkeley National Laboratory, DOE Joint Genome Institute le Univesithi ea California, Berkeley.